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 Ph: 877-853-9755
 Fax: 866-533-7490
 Email: info@biosciencetools.com


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Product news - recently upgraded items:


Objective heater CO2 Mixing Chamber, $395
Designed to provide CO2 control inside small custom chambers and micro-incubators. The mixing chamber dimensions are 100x100x32mm, which are small enough to fit on a microscope stage. The chamber has x4 ports to connect multiple micro-incubators and one input from CO2 controller. After mixing to the required concentration, the gas mixture can be pumped into the micro-incubator using an optional pump or the miniature flow controller, that can deliver from micro-liters to 22ml/min of the gas flow.


Objective heater 1-Channel Programmable Pressure Pico-Injector - Spritzer and aspirator, $995
For use with pipettes, thin tubing, and small volume delivery systems. Connects to an external pressure source up to 100PSI for injection and to a vacuum source (100PSI max) for aspiration. Built-in programmable timers (100ms min). Manual push button. Includes a foot pedal control for easy operation.


Time lapsed imaging Miniature incubators - CO2, hypoxia (O2) and temperature controlled microscope stages
Extended variety of heated mini-incubators for long term time lapse imaging of live cell samples to fit on any microscope. Options for any size Petri dishes, slides, coverslips and custom fluidics devices are available. Improved CO2 and hypoxia controller. Upgraded easy to use high stability precision 2-channel temperature controller TC-1-100i with rs232 port (can be used with USB adapter).


miniature peristaltic pumps Programmable dosing and flow control pumps
New computerized miniature peristalitic pumps and multi-channel automated pumps can be programmed as precision dosing units or as injectors with precise timing protocol (including continuous loops). The units can be also controlled using digital and analog signals, manual remote, or through a computer rs232 port.


Automated liquid handling Programmable solution perfusion/injectors/spritzers with precision timers
Complete 8/16-channel systems based on a computerized valve controllers with optional built-in pressure pumps. Front touch display allows to program up to 16 step sequences (32 step if automatic "wash" is used), including continuous loops. This is an ideal instrument to generate automated dose-response curves or any other protocols that require automatic liquid handling. The systems can be also controlled using digital and analog signals, manual remote, or through a computer rs232 port.


Cooling stages Small cooling/heating stage to fit any mechanical or motorized microscope
New temperature controlled 128x86mm stage is the size of a standard multi-well plate and can be used to cool or heat samples up to 3x1in. Connects to upgraded BTC-1-100/BTC-2-100 1-channel or 2-channel temperature controller with rs232 interface. Can be also used with our high stability precision programmable temperature controller that can generate temperature complex cycles.


microscope objective cooling Objective cooling
We can also make an objective cooler to fit any microscope objective, according to drawings provided. The objective cooler/heater can be used with either 1-channel or 2-channel temperature controller.


Precision high stability pressure controller
Our new PC-R15/10 pressure controller has stability better than 0.5mmHg, which can be used to provide smooth liquid flow in sensitive microfluidics applications. In combination with PC-16 programmable 16-channel valve controller with precision timers, the customers are now able to build flexible automated liquid delivery and perfusion setups with precise flow control. Both pressure and valves controllers, including programmable spritzers have rs232 port for computer control.

Custom design and manufacturing
We can also produce custom computerized and mechanical items to meet your needs and requirements.



BIOSCIENCE TOOLS
Ph: 877-853-9755,
Fax: 866-533-7490
Email info@biosciencetools.com
References
Temperature control:
130 Spectroscopic THz near-field microscope. Optics Express Vol. 16, Issue 5, pp. 3430-3438 (2008);
129 Infrared neural stimulation induces intracellular Ca2+ release mediated by phospholipase C. J Biophotonics Volume 11, Issue 2 February 2018;
128 Rhodamine B as an optical thermometer in cells focally exposed to infrared laser light or nanosecond pulsed electric fields. Biomedical Optics Express Vol. 6, Issue 10, pp. 4105-4117 (2015);
127 The Ubiquitin-like Protein PLIC-2 Is a Negative Regulator of G Protein-coupled Receptor Endocytosis. Mol. Biol. Cell March 1, 2008 vol. 19 no. 3 1252-1260;
126 IH current generates the afterhyperpolarisation following activation of subthreshold cortical synaptic inputs to striatal cholinergic interneurons. J Physiol 587.24 (2009) pp 5879–5897;
125 2,2,2-Trichloroethanol activates a non-classical potassium channel in cerebrovascular smooth muscle and dilates the middle cerebral artery. JPET December 2, 2009;
124 Directed self-assembly of liquid crystalline blue-phases into ideal single-crystals. Nature communications 2041-1723 Jun 2017 ;
123 Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry. Appl. Phys. Lett. 102, 193705 (2013);
122 Mesoscale martensitic transformation in single crystals of topological defects. PNAS September 5, 2017 11207;
121 Division site selection linked to inherited cell surface wave troughs in mycobacteria . Nature Microbiology VOLUME: 2 ARTICLE NUMBER: 17094 2017;
120 The Functional Activity of the Human Serotonin 5-HT1A Receptor Is Controlled by Lipid Bilayer Composition. Biophysical J. Volume 110, Issue 11, 7 June 2016, Pages 2486-2495;
119 Innocuous warming enhances peripheral serotonergic itch signaling and evokes enhanced responses in serotonin-responsive dorsal horn neurons in the mouse. J. Neurophysiology Volume 117,Issue 1 January 2017;
118 Fluorescence Lifetime Microscopy of Tumor Cell Invasion, Drug Delivery, and Cytotoxicity. Methods in Enzymology Volume 504, 2012, Pages 109-125;
117 Using DNA strand displacement to control interactions in DNA-grafted colloids. Soft Matter, 2018, 14, 969;
116 Detection of Site-dependent Segmental Mobility of Polymer. Journal of Polymer Science, 2017, 35(12): 1488-1496;
115 CbtA toxin of Escherichia coli inhibits cell division and cell elongation via direct and independent interactions with FtsZ and MreB. PLoS Genet 13 (9) 2017;
114 EphA2 Expression Regulates Inflammation and Fibroproliferative Remodeling in Atherosclerosis. Circulation. 2017;136:566-582;
113 Effect of suberoylanilide hydroxamic acid (SAHA) on breast cancer cells within a tumor–stroma microfluidic model. Integrative Biology 12, 2017;
112 Biocompatibility assay of cellular behavior inside a leaf-inspired biomimetic microdevice at the single-cell level. RSC Adv., 2017, 7, 32710;
111 Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells. Int J Radiat Oncol Biol Phys. 2016 Sep 1;96(1):221-7;
110 Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment. Sci Rep. 2016; 6: 34094.;
109 Three dimensional time-gated tracking of non-blinking quantum dots in live cells;
108 Examining dynamics in a polymer matrix by single molecule fluorescence probes of different sizes. Soft Matter, 2016,12, 7299-7306;
107 Role of T-Type calcium channels in a cellular model of stroke;
106 Enhanced Directional Migration of Cancer Stem Cells in 3D Aligned Collagen Matrices. Biophysical Journal Volume 112, Issue 5, p1023–1036, 14 March 2017;
105 Vascular Dysfunction in Pneumocystis-Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration. The American Journal of Pathology, Vol. 186, No. 2, February 2016;
104 Eugenol and carvacrol excite first- and second-order trigeminal neurons and enhance their heat-evoked responses. Neuroscience. 2014 June 20; 271: 45–55;
103 Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) and Endolysosomal Two-pore Channels Modulate Membrane Excitability and Stimulus-Secretion Coupling in Mouse Pancreatic ß Cells. The Journal of Biological Chemistry 290, 21376-21392. August 28, 2015;
102 Sensing cisplatin-induced permeation of single live human bladder cancer cells by scanning electrochemical microscopy . Analyst, 2015,140, 6054-6060;
101 Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes. Nat Commun. 2016; 7: 10312;
100 Bacillus subtilis chromosome organization oscillates between two distinct patterns. PNAS September 2, 2014 vol. 111 no. 35;
99 Global Deletion of Ankrd1 Results in a Wound-Healing Phenotype Associated with Dermal Fibroblast Dysfunction. The American Journal of Pathology, Vol. 185, No. 1, January 2015;
98 Mitochondrial Ca2+ Uptake from Plasma Membrane Cav3.2 Protein Channels Contributes to Ischemic Toxicity in PC12 Cells. Science Open, 18 March 2013;
97 MICROSTRUCTURE AND PHASE BEHAVIOR IN COLLOIDS AND LIQUID CRYSTALS. University of Pennsylvania;
96 COMPOUNDS THAT BIND DYSTROGLYCAN AND USES THEREOF. United States Patent Application 20150374845;
95 Molecular Architecture of Synaptic Actin Cytoskeleton in Hippocampal Neurons Reveals a Mechanism of Dendritic Spine Morphogenesis. Mol. Biol. Cell January 1, 2010 vol. 21 no. 1 165-176;
94 Effects of cytoskeletal disruption on transport, structure, and rheology within mammalian cells. PHYSICS OF FLUIDS 19, 103102 2007;
93 Vector frequency-comb Fourier-transform spectroscopy for characterizing metamaterials. New Journal of Physics, Volume 10, December 2008;
92 Eugenol and carvacrol excite first- and second-order trigeminal neurons and enhance their heat-evoked responses. Neuroscience. 2014 June 20; 271: 45–55;
91 REGAINING TAXOL SENSITIVITY IN CHEMORESISTANT OVARIAN CANCER CELLS. Georgia Institute of Technology May 2015;
90 Scanning Electrochemical Microscopy of Individual Pancreatic Islets. Journal of The Electrochemical Society, 163 (4) H3077-H3082 (2016);
89 Droplet-based electro-coalescence for probing threshold disjoining pressure. Lab on a Chip, 2015, v. 15 n. 9, p. 2018-2024;
88 Global Deletion of Ankrd1 Results in a Wound-Healing Phenotype Associated with Dermal Fibroblast Dysfunction. The American Journal of Pathology Volume 185, Issue 1, January 2015, Pages 96–109;
87 Rhodamine B as an optical thermometer in cells focally exposed to infrared laser light or nanosecond pulsed electric fields. BIOMEDICAL OPTICS EXPRESS 4105 2015 Vol. 6, No. 10;
86 A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement. Nature Communications 6, Article number: 8496, 29 September 2015;
85 Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control. JOVE 3/30/2015, Issue 97;
84 Apparatus and Method for Producing Specimens for Electron Microscopy. patent US 20140360286 A1;
83 Evolution of Network Synchronization during Early Epileptogenesis Parallels Synaptic Circuit Alterations. The Journal of Neuroscience, 8 July 2015, 35(27): 9920-9934;
82 Single-Molecule Imaging Reveals that Argonaute Reshapes the Binding Properties of Its Nucleic Acid Guides Cell. Cell 2015 vol.162 issue 1, p84-95;
78 Real-Time Imaging of Discrete Exocytic Events Mediating Surface Delivery of AMPA Receptors. The Journal of Neuroscience, 10 October 2007, 27(41): 11112-11121;
81 Potentiation of NMDA receptor-mediated transmission in striatal cholinergic interneurons. Front. Cell. Neurosci., 09 April 2015;
80 Comprehensive analysis of mitochondrial permeability transition pore activity in living cells using fluorescence-imaging-based techniques. Nature Protocols 11, 1067–1080 (2016);
79 Measurement of DCF fluorescence as a measure of reactive oxygen species in murine islets of Langerhans. Anal. Methods, 2014,6, 3019-3024;
78 Conformational biosensors reveal adrenoceptor signalling from endosomes. Nature. 2013 Mar 28; 495(7442);
77 Real-Time Imaging of Discrete Exocytic Events Mediating Surface Delivery of AMPA Receptors. The Journal of Neuroscience, 10 October 2007, 27(41): 11112-11121;
76 Spectroscopic THz near-field microscope. Optics Express Vol. 16, Issue 5, pp. 3430-3438 (2008);
75 Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control. J. Vis. Exp. (97), e52620;
74 Capture and release of particles from liquid samples;
73 METHODS OF GENERATING ENGINEERED INNERVATED TISSUE AND USES THEREOF;
72 Live Imaging Kidney Development in Zebrafish. Methods in Molecular Biology Volume: 886 Pub. Date: May-31-2012 Page Range: 55-70;
71 Droplet-based electro-coalescence for probing threshold disjoining pressure. Lab Chip, 2015,15, 2018-2024;
70 Dopamine-Mediated Autocrine Inhibitory Circuit Regulating Human Insulin Secretion in Vitro. Mol Endocrinol. 2012 Oct; 26(10): 1757–1772;
69 Alternative Translation Initiation of Human Regulators of G-Protein Signaling-2 Yields a Set of Functionally Distinct Proteins. Molecular Pharmacology January 2008 vol. 73 no. 1 1-11 ;
68 Elastic Instability of a Crystal Growing on a Curved Surface. 7 February 2014, Science 343, 634 (2014);
67 Bacillus subtilis chromosome organization oscillates between two distinct pattern. PNAS September 2, 2014 vol. 111 no. 35 12877–12882;
66 The small GTPase Rap1 promotes cell movement rather than stabilizes adhesion in epithelial cells responding to insulin-like growth factor I. Biochem. J. (2014) 463 (257–270);
65 Potentiation of NMDA receptor-mediated transmission in striatal cholinergic interneurons. Front. Cell. Neurosci., 09 April 2015;
64 Visual-Induced Excitation Leads to Firing Pauses in Striatal Cholinergic Interneurons. The Journal of Neuroscience, 3 August 2011, 31(31): 11133-11143;
63 Connexin40 Imparts Conduction Heterogeneity to Atrial Tissue. Circ Res. 2008;103:1001-1008;
62 Investigating G protein-coupled receptor endocytosis and trafficking by TIR-FM. Methods Mol Biol. 2011; 756: 325–332;
61 Human sensory neurons: membrane properties and sensitization by inflamatory mediators. PAIN 155 (2014) 1861-1870;
60 Alterations in Ovarian Cancer Cell Adhesion Drive Taxol Resistance by Increasing Microtubule Dynamics in a FAK-dependent Manner. Nature, Scientific Reports, 17 April 2015, Volume:5, Article number:9529;
59 Measurement of DCF fluorescence as a measure of reactive oxygen species in murine islets of Langerhans. Anal. Methods, 2014,6, 3019-3024,
58a 58b Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry. Appl Phys Lett. 2013 May 13; 102(19): 193705,
57, 56, 55, 54, 53, 52, 51, 50, 49, 48, 47, 46, 45, 44, 43, 42, 41, 40, 39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10

Liquid delivery and perfusion:
40 A novel inwardly rectifying K+ channel, Kir2.5, is upregulated under chronic cold stress in fish cardiac myocytes. Journal of Experimental Biology 2008 211: 2162-2171;
39 Full mutational mapping of titratable residues helps to identify proton-sensors involved in the control of channel gating in the Gloeobacter violaceus pentameric ligand-gated ion channel. PLOS Biology December 27, 2017;
38 Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator. J. Vis. Exp. (126), e55698, doi:10.3791/55698 (2017);
37 Fusion of Mature HIV-1 Particles Leads to Complete Release of a Gag-GFP-Based Content Marker and Raises the Intraviral pH. PLoS ONE Volume 8, Number 8 2013;
36 Exocytotic release of ATP by preBötzinger Complex astrocytes contributes to the hypoxic ventilatory response via a calcium-dependent P2Y1 receptor mechanism;
35 Connectivity, Organization, and Network Coordination of the Drosophila Central Circadian Clock;
34 EFFECT OF ECONAZOLE AND BENZYDAMINE ON SENSORY NEURONS IN CULTURE. JPP No 6/2016 article 06;
33 Independent role for presynaptic FMRP revealed by an FMR1 missense mutation associated with intellectual disability and seizures . PNAS vol. 112 no. 4, 949–956;
32 Optogenetic Control of Gene Expression in Drosophila. PLOS one, September 18, 2015;
31 Spatiotemporal Coding of Individual Chemicals by the Gustatory System. The Journal of Neuroscience, 2 September 2015, 35(35): 12309-12321;
30 Evaluating the Functionality of Conjunctiva Using a Rabbit Dry Eye Model. Journal of Ophthalmology Volume 2016 (2016);
29 Visualization of retrovirus uptake and delivery into acidic endosomes. Biochemical Journal Mar 15, 2011, 434 (3) 559-569;
28 Basolateral and central amygdala differentially recruit and maintain dorsolateral striatumdependent cocaine-seeking habits. NATURE COMMUNICATIONS 14 Dec 2015;
27. Human sensory neurons: membrane properties and sensitization by inflamatory mediators. PAIN 155 (2014) 1861-1870;
26. Size-Dependent Cellular Uptake and Expulsion of Single-Walled Carbon Nanotubes: Single Particle Tracking and a Generic Uptake Model for Nanoparticles. ACS Nano, 2009, 3 (1), pp 149–158;
25. Analysis of functional neuronal connectivity in the Drosophila brain. J Neurophysiol. 2012 Jul 15; 108(2): 684–696;
24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 6, 7, 8, 9, 1, 2, 3, 4, 5

Sample and perfusion chambers:
41 2,2,2-Trichloroethanol activates a non-classical potassium channel in cerebrovascular smooth muscle and dilates the middle cerebral artery. JPET December 2, 2009;
40 Dissection of mechanical force in living cells by super-resolved traction force microscopy Nature Protocols volume 12, pages 783–796 (2017);
39 Pivotal Role of Actin Depolymerization in the Regulation of Cochlear Outer Hair Cell Motility Biophysical Jurnal Volume 99, Issue 7, 6 October 2010, Pages 2067-2076;
39 Improved Glass Surface Passivation for Single-Molecule Nanoarrays Langmuir, 2016, 32 (39), pp 10034–10041;
38 Transfection of Cells with DNA Encoding a Visible Fluorescent Protein-Tagged Lipid-Binding Domain Spring Harb Protoc 2017;
37 Pathogen-Related Differences in the Abundance of Presented Antigen Are Reflected in CD4+ T Cell Dynamic Behavior and Effector Function in the Lung. J Immunol published online 15 January 2014;
36 A neural network underlying circadian entrainment and photoperiodic adjustment of sleep and activity in Drosophila. Journal of Neuroscience, 36(35), 9084-9096. 2016;
35 Dissection of mechanical force in living cells by super-resolved ... Proc. Natl. Acad. Sci. USA 113, 2898–2903 (2016).;
34 Unique Hydrophobic Extension of the RGS2 Amphipathic Helix Domain Imparts Increased Plasma Membrane Binding and Function Relative to Other RGS R4/B Subfamily Members. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 282, NO. 45, pp. 33064–33075, November 9, 2007;
32 Eugenol and carvacrol excite first- and second-order trigeminal neurons and enhance their heat-evoked responses. Neuroscience. 2014 June 20; 271: 45–55;
31 Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) and Endolysosomal Two-pore Channels Modulate Membrane Excitability and Stimulus-Secretion Coupling in Mouse Pancreatic ß Cells. The Journal of Biological Chemistry 290, 21376-21392. August 28, 2015;
30 PKCa-Mediated Signals Regulate the Motile Responses of Cochlear Outer Hair Cells. Biophysical Journal Volume 108, Issue 9, 5 May 2015, Pages 2171–2180;
29 Dynamical feature extraction at the sensory periphery guides chemotaxis. eLife 2015;4:e06694;
28. Fluorescence coherence tomography. Optics Express Vol. 14, Issue 16, pp. 7134-7143 (2006);
27. Separation of absorption and scattering profiles in spectroscopic optical coherence tomography using a least-squares algorithm. Optics Express Vol. 12, Issue 20, pp. 4790-4803 (2004);
26. Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells. J. Vis. Exp. (84), e50985 (2014);
25. Alternative Translation Initiation of Human Regulators of G-Protein Signaling-2 Yields a Set of Functionally Distinct Proteins. Molecular Pharmacology January 2008 vol. 73 no. 1 1-11 ;
24. Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry. Appl Phys Lett. 2013 May 13; 102(19): 193705;
23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 1, 2, 3, 4, 5.

Petri dish perfusion:
15 Evaluation of Optogenetic Electrophysiology Tools in Human Stem Cell-Derived Cardiomyocytes. Frontiers in Physiology 8 October 2017;
14 A neural network underlying circadian entrainment and photoperiodic adjustment of sleep and activity in Drosophila. Journal of Neuroscience, 36(35), 9084-9096. 2016;
13 Optogenetic Control of Gene Expression in Drosophila. PLOS one, September 18, 2015;
12. Analysis of functional neuronal connectivity in the Drosophila brain J Neurophysiol. 2012 Jul 15; 108(2): 684–696;
11. Differentially timed extracellular signals synchronize pacemaker neuron clocks. PLoS Biol. 2014 September; 12(9);
10, 9, 8, 7, 6, 1, 2, 3, 4, 5.
CONTROLLED FLOW PERFUSION SYSTEM Perfusion Systems Flow Control Temperature Control Miniature Holders Miniature Manifolds Local Perfusion Small Volume Delivery System Perfusion Chambers for replaceable coverslip Ultra-Thin Imaging Chambers Closed Controlled Environment Chambers & Mini-Incubators Uniform Heating Slide Heaters Chambers, Thin profile, Heated, Perfusion, for AFM and Water Immersion Accessories Low Profile Heated Chambers Imaging, High Resolution Chambers Accessories Chambers with Transparent Uniform Heaters Heated Chambers Open Chambers Thin Sealed Chambers Magnetic & Screw-Type Holders, Miniature Perfusion Accessories, Micro-Manifolds and Tubing micro-Manifolds Miscellaneous Perfusion Pumps Small Volume Delivery Systems Tubing & Fitting Perfusion Chambers Controlled Environment Closed Imaging Chambers CoverSlip Chambers Petri Dish Inserts Perfusion Systems, Fast and Miniature Pinch Valves Perfusion Systems Perfusion Systems, Flow Control Accessories Miniature Perfusion Systems Pressure Micro Injectors - Spritzers 1-Channel Injectors 4, 8-channel Micro Injectors - Spritzers Temperature Controllers, Heating 1-Channel Controller, Low Electrical Noise 2-Channel Temperature Controller Temperature Controllers, Heating & Cooling Bipolar Heating/Cooling Controllers