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Heating stage for glass coverslips, TC-CSC
Ready to use system for samples cultured/placed on
coverslips. Used with bath chambers for replaceable coverslips, CSC.
Replaceable slips allow to culture cells before performing
experiments. Keeps temperature steady even if used with perfusion
systems. The heater preheats perfusion solution before it enters the chamber. The bottom part of the chamber is aluminum to
facilitate heat transfer. The glass
is clamped with O-ring to isolate solution from the metal bottom.
Inline heated Teflon tubing fits
manifolds included with perfusion systems. Includes
a miniature holder to position the temperature probe anywhere around your
sample.
Can be used
for imaging
and fluorescence
as well as recording.
The open chamber design offers the advantages of using with water
immersion optics of upright microscopes, fluorescence imaging with
inverted microscopes, option to apply substances locally through
miniature manifolds,
and ability to perform electrophysiological recordings. Can be used
with 35 mm petri dishes. Consider adding a miniature
and controlled
flow perfusion systems. Requires a microscope adapter. Consider adding an outflow
unit for suction to remove solution from the chamber during
perfusion. Requires a temperature controller.
Click on image to enlarge.
Sample publication on using
the heating stage.
These temperature controlled systems are
also referenced in Confocal Microscopy of Living Cells
tecnhiques.
Sample movies obtained from live transgenic embryo samples incubated
using the heating stage.
Produced by Albert D. Kim, Julien Y. Bertrand from David Traver's Lab, UCSD, San Diego, CA
Heating Element with 35mm clearance, TC-E35
Fits 35mm dishes and chambers. Whole bottom is open, which makes it ideal for
replaceable coverslip chambers. The heating element
incorporates replaceable Teflon perfusion tubing inside,
which makes the element to work as inline preheater. Since some brands of petri
dishes have different diameter, reducing adapters TC-PA might be required.
Requires a microscope adapters with 50mm
mounting opening. Requires a temperature controller. This element is a part of TC-CSC heating
stages. Click on image to enlarge.
Specifications: |
Dimensions: | 52mm
diameter |
Temperature stability: | <0.1°C, built-in sensor |
Optical clearance: | 35mm |
Use with: | Coverslips and Petri dishes, including 35mm glass bottom dishes |
Solution Pre-heater: | Replaceable/Removable Teflon tubing, easy to wash |
Microscope adapter: | Fits to 50mm cutout of standard microscope adapters |
Click on catalog numbers below to purchase online.
Required accessories: temperature controllers,
microscope adapters,
coverslip chambers.
Optional accessories: objective heater,
miniature holders,
perfusion system,
flow control.
Click on catalog numbers below to purchase online.
Download PDF catalog.
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Bioscience Tools
ph: 1-877-853-9755
ph: 1-858-537-1062 (from outside USA)
fax: 1-619-582-0919
email: info@biosciencetools.com
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PRICES AND OPTIONS
Temperature Controlled Chambers and Perfusion |
TC-CSC |
$95 |
Heater for chambers for replaceable coverslips
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CSC |
$95 |
Round Coverslip Holder Specify coverslip diameter when ordering.
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TC-PA |
$90 |
Reducing adapter-ring, set of 3. Allows you to use different brand dishes.
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TC-E35 |
$54 |
Replacement Heating Element with 35mm clearance.
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