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Miniature Incubator, TC-MWP
Designed for long-term live cell imaging and time-lapsed microscopy. It can be used with standard
multi-well plates and optional inserts: TC-I-20x30, TC-I-30x50, TC-I-SL for slides and
micro-fluidics devices, TC-I-35/TC-I-60 for standard 35mm Petri
dishes and for larger dishes (up to 60mm
diameter), TC-I-3 - for slides
and chambered coverglasses (x3), and TC-I-4 - for CSC coverslip holders and
Petri dishes (x4).
The inserts can be used with a set of adjustable tubing
holders MH-MIS to position sensors or perfusion tubing for
continuous media exchange. Requires a heated cover (purchased separately) to prevent
condensation and to control CO2 or hypoxia. Built-in multiple ports for
tubing and accessories, probes and sensors. Fits all brands of motorized
stages (Ludl, Prior, ASI, Marhauser) and type K Zeiss/Leica stages.
Some models of motorized stages from Nikon, ThorLabs and Ludl might
require optional adapter extensions to fit (see the table below).
Requires a temperature controller.
The
whole bottom is open for access with a microscope objective (for closed heated bottom
incubator - consider TC-MWPHB model).
Can be upgraded with an objective heater for immersion optics.
Sample publications:
7
Redox differences between rat neonatal and adult cardiomyocytes under hypoxia.
Free Radical Biology and Medicine, Volume 211, 1 February 2024, Pages 145-157;
6
Hydrogen peroxide is not generated intracellularly in human neural spheroids during ischemia-reperfusion.
Free Radical Biology and Medicine Volume 212, 20 February 2024, Pages 234-240;
5
Hyperglycemia exacerbates ischemic stroke not through increased generation of hydrogen peroxide.
Free Radical Biology and Medicine, Volume 208, 1 November 2023, Pages 153-164;
4
Integrated functional neuronal network analysis of 3D silk-collagen scaffold-based mouse cortical culture.
STAR Protocols Volume 2, Issue 1, 19 March 2021, 100292;
3
In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model.
j.redox.2021.102178;
2
A Human Organotypic Microfluidic Tumor Model Permits Investigation of the Interplay between Patient-Derived Fibroblasts and Breast Cancer Cells.
Cancer Research April 16, 2019;
1
Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment.
Sci Rep. 2016; 6: 34094.;
Brand |
extension (if required) |
Ludl, Prior, ASI, Marhauser,
Zeiss |
no extension required |
Nikon 236x155mm: |
TC-MI-NIK |
Thorlabs 170x130mm: |
TC-MI-THOR |
Ludl Bioprecision II, 172x116mm: |
TC-MI-LUDL |
Specifications: |
Outside dimensions: | fits
160x110mm cutout of motorized stages; the bottom is recessed 11mm below the top surface
of motorized stages (8mm below the mounting surface, comes with 3mm spacers to
reduce this recessed profile to 5mm), and can be leveled by set screws positioned in the corners of the incubator base |
Optical aperture and
clearance: | 114x76mm aperture;
33mm from bottom to top lid surface (26.5mm for ultra-low lid); 26mm inside
clearance (19.5mm for ultra-low lid); optional spacers allow to elevate the top surface 9.5mm - to provide more space inside the incubator |
Use with: | Standard
multi-well plates, 35-60mm dishes, custom devices and 1x3in. glass coverglasses/slides |
Stability: | 0.01°C |
Condensation free lid: | Uses
a built-in temperature sensor to connect independently to
the second channel of temperature controllers, 81x121mm optical window |
CO2 port: | Luer-lock
or barbed connector to a source of gas mixture. |
Media exchange and perfusion: |
Multiple sealed inflow/outflow ports,
optional sets of adjustable tubing holders to position inflow and outflow tubing inside sample chambers. |
Magnetic insert, MA-128x86
This insert provides flexible working area for positioning accessories
required for high resolution live sample imaging and recording: from media exchange and
test solution delivery tubing, to sensors and electrodes. Specially treated stainless
magnetic surface of the adapter provides ideal means to mount miniature
adjustable magnetic holders.
Shown on the picture are ZMM zero-dead volume manifold and MTH-S holder with
stainless suction tubing. Incorporates adjustable clamps to fix
all brands of 35mm Petri dishes and CSC chambers, glass bottom dishes
(both 35 and 50mm), and heating elements. Click on image to enlarge.
Insert for standard slides, TC-I-20x30
Designed to hold standard 1x3in. slides. Provides wide access for fluidics
tubing.
Optical aperture is 20x30mm, 1mm thick lip to hold slides up to 76x26mm. Includes adjustable clumps and thumb screws.
Click on image to enlarge.
Insert for slides and microfludics devices, TC-I-SL
Designed to position custom microfluidics devices and slides. Provides wide access for fluidics
tubing - 80x70mm recessed area.
Optical aperture is 72x24mm, 1mm thick lip to hold slides up to 76x28mm. Includes adjustable clumps and thumb screws.
Click on image to enlarge.
Insert for custom devices, TC-I-30x50
Designed to hold custom devices up to 101x51mm. Provides wide access for custom accessories.
Optical aperture is 50x30mm. Includes adjustable clumps and thumb screws.
Click on image to enlarge.
Insert for 35mm dishes, TC-I-35
Designed to position standard 35mm Petri dishes. Provides wide access for custom accessories and fluidics tubing.
Optical aperture is 25mm. Includes adjustable clumps and thumb screws.
Insert for 50-60mm dishes, TC-I-60
Designed to position larger (up to 60mm diameter) dishes. Provides wide access for custom accessories and fluidics tubing.
Optical aperture is 30mm. Includes adjustable clumps and thumb screws.
Insert for x4 coverslip holders and Petri dishes, TC-I-4
Designed to hold x4 CSC coverslip holders and Petri dishes (up to 36.8mm diameter) dishes.
Some brands of Petri dishes require reducing rings (TC-PA-C, -F, -W, -N). Provides wide access for custom accessories.
Optical aperture is 34mm. Includes adjustable clumps and thumb screws.
Insert for x3 slides/coverglsses, TC-I-3
Designed to hold x3 coverglasses and custom slides. Optical aperture is 70x20mm.
Click on catalog numbers below to purchase online.
Required accessories: temperature controller,
CO2 controller.
Optional accessories: objective heater,
perfusion system and accessories,
flow control.
Download PDF manual.
Download PDF catalog.
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Bioscience Tools
ph: 877-853-9755, fax: 866-533-7490
email: info@biosciencetools.com
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PRICES AND OPTIONS |
TC-MWP |
$1,395 |
Miniature Incubator for multi-well plates
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TC-MWPL |
$1,395 |
Lid for miniature Incubator TC-MWP, -MWPHB, -MIW
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TC-MWPLL |
$1,395 |
Ultra-low lid for miniature Incubator TC-MWP,
-MWPHB, -MIW
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TC-MI-THOR |
$295 |
Adapter for ThorLabs stages, 170x130mm
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TC-MI-NIK |
$295 |
Adapter for Nikon motorized stages, 236x155mm
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TC-MI-LUDL |
$295 |
Adapter for Ludl Bioprecision II stages.
172x116mm
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TC-I-20x30 |
$295 |
128x86mm insert for standard slides, 20x30mm aperture
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TC-I-30x50 |
$295 |
128x86mm insert for custom devices, 50x30mm aperture
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TC-I-SL |
$295 |
Insert for slides and microfludics devices,
24x72mm aperture |
TC-I-60 |
$295 |
Insert for 50-60mm dishes |
TC-I-35 |
$295 |
128x86mm insert for 35mm dishes
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MA-128x86 |
$295 |
Magnetic insert, stainless steel, 128x86mm
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TC-I-4 |
$295 |
A metal insert, to position adjustable holders, can be used with x4 dishes and chambers
up to 38mm diameter, reducing adapters might be required for different brands of dishes |
TC-I-3 |
$295 |
A metal inserts, to position adjustable holders, can be used with slides and coverglasses |
MH-MIS |
$295 |
Set of miniature adjustable tubing and sensor holders, x3, includes 4-40 threaded posts
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TC-I-E |
$295 |
Spacer to elevate the lid 9.5mm
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TC-PA-C |
$95 |
Reducing adapter-ring, for Corning type dishes.
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TC-PA-N |
$95 |
Reducing adapter-ring, for Nunc type dishes.
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TC-PA-W |
$95 |
Reducing adapter-ring, for Willco dishes.
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TC-PA-F |
$95 |
Reducing adapter-ring, for FluoroDishes.
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