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Low Profile Perfusion Set for Petri dishes
Comes with PDI insert and 50
self-adhesive biocompatible gaskets,
which allow to use the assembly
with regular 35 mm Corning petri dishes. Download PDF brochure.
Cells cultured in 35 mm Petri dishes are a popular research tool used in
numerous applications, including patch clamping and intracellular ion probe
imaging. However, true perfusion (continuous inflow and outflow) of solutions
can be difficult to configure. Drug delivery without an outflow requires a
spritzer-type microinjector, but ultimately results in contamination of the
entire dish after only a few applications. This forces scientists to plate cells
on cover slips for placement into specially designed perfusion chambers.
However, such transfer is a time consuming process which introduces the
potential for contamination plus additional expense.
The PDI chamber was
designed by scientists after several years of patch clamp research combined with
external perfusion of single cells cultured in Petri dishes. The chamber has
separate openings for solution inflow and outflow that dump the fluctuations of
the liquid level in the working compartment and prevents bubbles
from entering the chamber. The laminar profile facilitates perfusion and provides faster solution exchange.
An adjustable metal suction tube (included) controls the level of liquid in the dish. The chamber was designed for use with
perfusion systems
and included magnetic holder with miniature ball-joint can accommodate
perfusion manifolds
(optional). Can be
used with glass bottom dishes
for imaging. In fact, some glass bottom dishes are made from standard petri
dishes like Corning 35 mm, for example (call for details.) Can be used with temperature controlled systems.
The suction tubing requires connection to an outflow source CFPS-1U.
See published sample recordings,
and sample publications
1,
2,
3,
4,
5.
Includes three magnetic tubing/electrode holders, and stainless
suction tubing. A microscope adapter
is required. Can be used with both magnetic and
non-magnetic microscope adapters.
If used with non-magnetic adapters, a IMA-MH set of miniature holders
is required. Click on image to enlarge.
Specifications: |
Outside diameter: | 35mm |
Height: | 3mm |
Working volume: | 11mm diameter, approx. 100 microl |
Click on catalog numbers below to purchase online.
Required accessories: microscope adapter.
Optional accessories: perfusion system,
temperature control,
flow control,
miniature tubing holders.
Download PDF manual.
Download PDF catalog.
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Bioscience Tools
ph: 1-877-853-9755
ph: 1-858-537-1062 (from outside USA)
fax: 1-619-582-0919
email: info@biosciencetools.com
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PRICES AND OPTIONS
Petri
Dish Inserts |
LPPCP1 |
$225 |
Low Profile Perfusion System for Petri dishes.
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MA |
$75 |
Magnetic microscope adapter, specify microscope model when ordering.
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PDI |
$95 |
Replacement Low Profile Chamber-Insert for Petri dish |
IMA |
$75 |
Universal microscope adapters, specify microscope model when ordering.
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IMA-MH |
$110 |
Miniature holders set for univesal adapters IMA, x3.
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TC-PCP-15 |
$410 |
Temperature Controlled Petri dish perfusion chamber with magnetic holders |
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