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2-Channel Ultra Fast Temperature Change System, TC2-UF
This patented system is based on our
ultra-fast perfusion system
(below) and utilizes two square profile heated capillaries. Capillaries are
mounted on a special 2-channel holder, which allows you to position the
capillaries close to each other and at a required angle. Initially, a sample is
submerged in one flowing solution. Fast temperature switch happens after turning
one solution flow off. As a result, the sample is submerged to the second
solution at a different temperature. Because of small size of the sample and
thin gradient between two flowing solutions, a rate of 200 microseconds could be
achieved using this method. The system includes 2-channel 1.5 ms switch rate
solenoid valve perfusion system, 2-channel temperature controller, 2-capillary
holder-manipulator, four 1x1mm 10mm long capillaries (two of them have heating
element around, and a built in thermistor.) Consider adding additional
manipulator to
mount the capillaries holder. Click on image to enlarge.
Download QuickTime movie.
Spare Heated Capillary, 1x1mm, TC2-UF-S
Has built-in thermistor and a connector for the cable from the temperature controller
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Ultra-fast solution switch system for single cells, PSUF1
This system is based on manipulation the interface between two solutions. Since
there no mechanical movement around the cells is involved, sub-millisecond rates
could be achieved. This also makes the system very gentle on the cells because
speed of the solutions surrounding the cells does not change. Two solutions are
constantly flowing from two square 1 x 1 mm tubing forming an interface, which
moves if the flow of one of the solutions stops.
Initially the cell (or
patch) is inside the first solution, which is usually the same as the bath
(control) solution. After the flow of the first solution stops, the second
solution takes its place. As a result the cell becomes immersed inside the
second solution. To go back to the control solution, turn the second solution
flow OFF (or turn the first solution ON again), and the cell becomes immersed
into the bath (control) solution. Click on image to see the schematics of
the solution switch. Since the size of the cell is small the solution interface
moves across the cell surface very rapidly. Exchange rates of 0.2 ms have been
achieved using this technique.
The system includes a two pipette holder
with tilting, pivot and one-axis movement controls, which allows to adjust two
square outflow tubing relative to each other. Two straight 100 mm long and two
bent borosilicate glass square tubing are included. Bent at an angle tubing
allows to use the system under water immersion objectives because the pipette
holder could be position at a distance from the sample.
Includes 2-channel miniature fast perfusion system,
thin polyimide tubing to put inside the capillaries, and
polyethylene tubing that fits over the capillaries. Could be used with gravity
driven perfusion lines. Consider adding a coarse manipulator
to mount the pipettes holder, a small reservoirs system
or a syringe holder.
Click to see enlarged
picture.
Because of continuous solutions flow, which might result to
contamination of the bath solution, it is desirable to washout the bath chamber
constantly. Consider upgrading this system for additional valves for bath
perfusion. Bath perfusion chambers are
also available.
Square Borosilicate Tubing, 10 pack, PSUF-CAP
Square glass tubing for ultra-fast perfusion system. Outside dimensions 1x1 mm,
Inside dimensions 0.67x0.67 mm. 100 mm long. Could be bent at an angle after
heating over a burner.
Click on catalog numbers below to purchase online.
Download PDF catalog.
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Bioscience Tools
ph: 1-877-853-9755
ph: 1-858-537-1062 (from outside USA)
fax: 1-619-582-0919
email: info@biosciencetools.com
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PRICES AND OPTIONS
Ultra-Fast
Perfusion and Temperature Switch |
TC2-UF |
$4,900 |
2-Channel Ultra Fast Temperature Change System
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TC2-UF-S |
$225 |
Spare Heated Capillary, 1x1mm
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PSUF1 |
$3,360 |
Ultra-fast solution switching system |
PSUF-CAP |
$180 |
Square Borosilicate Tubing, 10 pack |
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