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Glass Bottom Dishes vs. Petri Dishes
Glass bottom dishes
are used with short working distance, high N.A. objective in fluorescence,
confocal and image analysis experiments. Polarization free, does not distort
Differential Interference Contrast. The outside shape and glass flat bottom
provides a parallel (optical) fit in the microscope stages and good thermal
conductivity in heated platforms (below.) The inside well allows you to use only
small volumes of media, if necessary: See schematics.
Click for glass coating procedures.
The heating elements with reduced optical window in the middle
(down to 15 or 11mm) will provide better temperature stability for
your sample, and are perfect for use with glass bottom dishes and
long-working-distance objectives. If immersion objectives are used,
however, this small window will limit access of the large immersion
objective to the whole bottom surface. In this case a heating element
with 35mm clearance/window TC-E35 should be used. Temperature
stability still can be provided by using an objective heater.
In contrasts to glass bottom dishes, which provide good thermal
contact because of flat glass bottom, standard plastic Petri dishes
very often have a protruding rim alone the bottom edges of the dish.
This rim elevates the dish above heating surface. Metal shims/washes
can be used to eliminate this air gap, however. This will reduce access for the immersion optics to the bottom of the dish, so this can
be used with long-distance objectives only. If using immersion optics and large objectives, 35mm heating element
TC-E35 should be used,
combined with
an objective heater.
Please note, that some
glass bottom dishes, Mattek dishes for example, are
fabricated from standard Corning (or Falcon dishes).
Since different brands of dishes have different outside diameter,
sometimes larger than 38mm, we make reducing inserts to provide better
fit and thermal contact to the inside cutout of the heating elements,
which is 38mm. We also make a 50mm insert to center the dishes inside
incubators and larger heating elements, which have 50mm inside cutout.
Click on catalog numbers below to purchase online.
Download PDF catalog.
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Bioscience Tools
ph: 877-853-9755
fax: 866-533-7490
email: info@biosciencetools.com
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PRICES AND OPTIONS |
TC-PA-C |
$95 |
Reducing adapter-ring, for Corning type dishes, including Mattek dishes.
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TC-PA-N |
$95 |
Reducing adapter-ring, for Nunc type dishes.
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TC-PA-W |
$95 |
Petri Dish Adapter. Petri dish adapter -reducing ring- to use the controlled environment setup with different brand petri dishes. The adapters fit inside the heater and fits around the 35mm
WillCo dish to eliminate air gaps and to prevent dish movement. |
TC-PA-F |
$95 |
Reducing adapter-ring, for FluoroDishes.
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TC-PA-G |
$95 |
Reducing adapter -reducing rings-, for Greiner Bio-One dishes, glass bottom.
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TC-PA50 |
$95 |
50mm reducing adapter for 35mm dishes, to use
with TC-MIS, TC-MI incubators and TC-E50 heating elements. |
PDI |
$195 |
Low Profile Chamber-Insert for Petri dish |
PCP2-W |
$150 |
Glass Bottom WillCo Dish Insert.
Modification of PDI insert for standard petri dishes. Facilitates perfusion inside the dish.
Separate inflow and outflow compartments prevent bubbles from entering
the working compartment and provide smooth perfusion. Can be used with
perfusion
systems. See sample recordings.
Choose dish size when ordering (35 mm dish with 12 mm.) |
LPPCP1-W |
$275 |
Glass Bottom WillCo Dish Perfusion Setup. In addition to the
PCP2 insert, includes a set of magnetic holders and
stainless suction tubing. Choose microscope adapter when ordering. |
W-HLD |
$195 |
Petri Dish Magnetic Clamps. These two clamps could be positioned anywhere on the stainless steel microscope adapter of the setup above and fix the dish firmly in place.
Can be used to prevent dish elevation while working with oil immersion objectives. |
MA |
$295 |
Magnetic microscope adapter, specify microscope model when ordering.
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