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Petri Dish Inserts > Low Profile Perfusion Set for Petri Dishes

Low Profile Perfusion Set for Petri dishes, LPPCP1

Comes with PDI insert and 50 self-adhesive biocompatible gaskets, which allow to use the assembly with regular 35 mm  Petri dishes.

Cells cultured in 35 mm Petri dishes are a popular research tool used in numerous applications, including patch clamping and intracellular ion probe imaging. However, true perfusion (continuous inflow and outflow) of solutions can be difficult to configure. Drug delivery without an outflow requires a spritzer-type microinjector, but ultimately results in contamination of the entire dish after only a few applications. This forces scientists to plate cells on cover slips for placement into specially designed perfusion chambers. However, such transfer is a time consuming process which introduces the potential for contamination plus additional expense.
The PDI chamber was designed by scientists after several years of patch clamp research and calcium imaging combined with external perfusion of single cells cultured in Petri dishes. The chamber has separate openings for solution inflow and outflow that dump the fluctuations of the liquid level in the working compartment and prevents bubbles from entering the chamber. The laminar profile facilitates perfusion and provides faster solution exchange. An adjustable metal suction tube (included) controls the level of liquid in the dish. The chamber was designed for use with perfusion systems and included magnetic holder with miniature ball-joint can accommodate perfusion manifolds (optional). Can be used with glass bottom dishes for imaging. In fact, some glass bottom dishes are made from standard Petri dishes like Corning 35 mm, for example (Mattek dishes.) Can be used with temperature controlled systems. The suction tubing requires connection to an outflow source CFPS-1U. See published sample recordings, and Sample publications.

Includes three magnetic tubing/electrode holders, and stainless suction tubing. A magnetic microscope adapter is required. Can be used with both magnetic and non-magnetic microscope adapters. If used with non-magnetic adapters, a IMA-MH set of miniature holders is required.

Specifications:

Outside diameter:

35mm

Height:

3mm

Working volume:

11mm diameter, approx. 100 microl



Click on catalog numbers below to purchase online.

Required accessories: microscope adapter, miniature tubing holders.

Optional accessories: perfusion system, temperature control, flow control.

Download PDF manual.

Download PDF catalog. Download PDF brochure.

Bioscience Tools
ph: 877-853-9755
fax: 866-533-7490
email: info@biosciencetools.com


PRICES AND OPTIONS

Petri Dish Inserts

LPPCP1

$475

Low Profile Perfusion System for Petri dishes.

MA

$295

Magnetic microscope adapter, specify microscope model when ordering.

PDI

$195

Replacement Low Profile Chamber-Insert for Petri dish

IMA

$195

Universal microscope adapters, specify microscope model when ordering.

IMA-MH

$295

Miniature holders set for univesal adapters IMA, x3.


See published sample recordings, and sample publications:
17 Enhancing Evanescent Wave Coupling of Near-Surface Waveguides with Plasmonic Nanoparticles. Sensors 2023, 23, 3945;
16 Sites of Circadian Clock Neuron Plasticity Mediate Sensory Integration and Entrainment. 2021;
15 Evaluation of Optogenetic Electrophysiology Tools in Human Stem Cell-Derived Cardiomyocytes. Frontiers in Physiology 8 October 2017;
14 A neural network underlying circadian entrainment and photoperiodic adjustment of sleep and activity in Drosophila. Journal of Neuroscience, 36(35), 9084-9096. 2016;
13 Optogenetic Control of Gene Expression in Drosophila. PLOS one, September 18, 2015;
12. Analysis of functional neuronal connectivity in the Drosophila brain J Neurophysiol. 2012 Jul 15; 108(2): 684–696;
11. Differentially timed extracellular signals synchronize pacemaker neuron clocks. PLoS Biol. 2014 September; 12(9);
10, 9, 8, 7, 6, 1, 2, 3, 4, 5.