Temperature controller
CO2/O2 control
Objective Heaters
Media Exchange
Solution Applications
Petri Dish Perfusion
Coverslip Chambers

Miniature Microscope Incubators > Incubator for Motorized Stages

Miniature Incubator for motorized and type K microscope stages, TC-MI-20x46

Designed for live cell imaging and time-lapsed microscopy. Can be used for long-term imaging: see cell chemotaxis tracks obtained during 8 hours incubation. This incubator can be used with 50mm dishes and chambers, slides/coverglasses, and, in combination with 50mm reducing inserts TC-PA50 (and TC-PA-C, -N, -F, -W), with 35 dishes and coverslip holders CSC and UTIC. Incorporates an inside reservoir for water to control humidity. Requires a heated lid (purchased separately) to prevent condensation and a to control CO2 and hypoxia. Built-in multiple sealed ports for tubing and accessories (probes and sensors). Can be upgraded with an objective heater for immersion optics. Fits all brands of motorized stages and Zeiss type K stages with 160x110mm insert (adapters for other stages are available, see the table below). An optional set of adjustable tubing holders MH-MIS allows you to position perfusion tubing for continuous media exchange. Requires a temperature controller. Sample publications:
1 Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry. Methods in Molecular Biology, 01 Jan 2021, 2304:315-337

For different size samples, consider TC-MWP incubator, which accepts various inserts designed specifically for multiple or larger samples: from 50-60mm dishes to four Petri dishes or four coverslip holders.


extension (if required)

Ludl, Prior, ASI, Marhauser, Zeiss

no extension required

Nikon 236x155mm:


Thorlabs 170x130mm:


Ludl Bioprecision II, 172x116mm:



Outside dimensions:

Fits 160x110mm cutout of motorized stages; the bottom is recessed 8mm below the mounting surface, comes with 3mm spacers to reduce this recessed profile to 5mm; can be leveled by set screws positioned in the corners of the incubator base

Optical window and clearance:

20x46mm aperture; 121x81mm top window; 33mm from bottom to top surface (26mm for low-profile); 26/19.5mm inside clearance (can be elevated using optional 9.5mm spacers)

Use with:

50mm and 35mm dishes, 1x3in. glass coverglasses/slides, coverslip holders CSC



Condensation free lid:

Uses a built-in temperature sensor to connect independently to the second channel of temperature controllers, 65x75mm optical window

CO2 port:

Luer connector to a source of gas mixture.

Water reservoir:

Controls humidity. Can be replenished through outside ports.

Media exchange and perfusion:

Multiple sealed inflow/outflow ports, an optional set of adjustable tubing holders to position inflow and outflow tubing inside sample chambers.

Adapter for ThorLabs stages TC-MI-THOR If attached to the bottom of incubators for motorized stages, allows you to fit the incubator inside 170x130mm cutout of ThorLabs stages. Includes two pieces for both sides.

Adapter for Nikon motorized stages TC-MI-NIK If attached to the bottom of incubators for motorized stages, allows you to fit the incubator inside 236x155mm cutout of Nikon motorized stages. Includes two pieces for both sides. Item#: TC-MI-NIK

Click on catalog numbers below to purchase online.

Required accessories: temperature controller, CO2/hypoxia controller.

Optional accessories: objective heater, perfusion system and accessories, flow control.

Download PDF manual.

Download PDF catalog.

Bioscience Tools
ph: 877-853-9755, fax: 866-533-7490
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Miniature Incubator for motorized stages



Low-Profile Lid for Miniature Incubators



Ultra-low lid for miniature Incubator TC-MWP, -MWPHB, -MIW



Spacer to elevate the cover 9.5mm (ships installed)



Adapter for ThorLabs stages, 170x130mm



Adapter for Nikon motorized stages, 236x155mm



Adapter for Ludl Bioprecision II stages, 172x116mm



Set of miniature adjustable tubing and sensor holders, x3, includes 4-40 threaded posts (two holders come with incubators)



Reducing adapter-ring, for Corning type dishes.



Reducing adapter-ring, for Nunc type dishes.



Reducing adapter-ring, for Willco dishes.



Reducing adapter  -reducing rings-, for FluoroDishes.



50mm reducing adapter-ring for 35mm dishes.