Temperature controller
CO2/O2 control
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Petri Dish Perfusion
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Miniature Microscope Incubators > CO2/O2 Controllers

CO2 controller for hypoxia experiments - CO2-O2-MI

Designed for live cell imaging and time-lapsed microscopy. For use with miniature incubators and custom enclosures. Connects to a cylinders with compressed CO2 gas and balance gas - Nitrogen usually. The output connects directly to a gas port of the incubator or a humidifier. The controller makes 0-20% CO2 and 0-20% O2 mixture to supply inside the incubator. CO2 control at 5% level keeps pH of media constant. Simple to use: the controller ships calibrated for 5% CO2 level requirement. Can be adjusted to fill incubators of different volumes. The instrument not only controls gas content inside the incubator, but also brings the gas consumption to the minimum. Click on image to enlarge.
Sample publication:
1. Intracellular oxygen mapping using a myoglobin-mCherry probe with fluorescence lifetime imaging J. Biomed. Opt. 23(10), 107001 (2018)



max 100PSI


750 sccm max


INPUT for 5/32in. (4mm) O.D. tubing (included);
OUTPUT for 1/8in O.D. tubing (included);
can be replaced with any 10-32 threaded connectors;
includes luer fitting for different size tubing;
includes easy-disconnect coupling for 1/4in. O.D. tubing


digital display of CO2, O2 and flow levels

Digital Controls:

CO2 level 0-20%

O2 level 0-20%

FLOW up to 750 sccm

INPUT PRESSURE manual regulators, 

CLOSE switches for CO2 and N2, 


Digital display


12 x 6 x 8in.


100-240VAC 25W

Heated humidifier CO2-500ml (Optional accessories)
A heated platform with 1L reservoir to provide gas saturation with water vapors by passing the gas mixture through heated water.

Required accessories: sources of CO2, N2 gases, miniature incubator.

Download PDF manual.

Download PDF catalog.

Click on catalog numbers below to purchase online.

Bioscience Tools
ph: 877-853-9755, fax: 866-533-7490




CO2/O2 Controller for Miniature Incubators



Heated Humidifier. When passed through heated water, CO2 mixture becomes humid. Can be also used as an indicator of gas flow - bubbles will indicated gas flowing inside the incubator. Connects inline between the controller and the incubator.